Enzymatic modification of hygromycin B in Streptomyces hygroscopicus.
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چکیده
منابع مشابه
Enzymatic modification of hygromycin B in Streptomyces hygroscopicus.
Antibiotic producing-organisms generally exhibit resistance to the antibiotics that they produce1). Such resistance mechanisms may take the form of target site modification, as is the case with erythromycin2) and thiostrepton3), or enzymatic modification of the antibiotic itself, as has been shown for a number of aminocyclitols4) The existence of these resistance mechanisms in producing organis...
متن کاملBiochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus--the producing organism.
Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by Streptomyces hygroscopicus. Ribosomes from this Gram-positive mycelial bacterium are inhibited in vitro by the antibiotic. In contrast, the streptomycete is highly resistant to the drug in vivo since it possesses hygromycin B phosphotransferase activity. This enzyme has been shown by g...
متن کاملPurification and characterization of a hygromycin B phosphotransferase from Streptomyces hygroscopicus.
A hygromycin B phosphotransferase activity from Streptomyces hygroscopicus has been highly purified by ammonium sulphate fractionation followed by affinity column chromatography through Sepharose-6B-hygromycin-B. The combined active fractions showed a single protein band (41 kDa) when subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. When gel electropho...
متن کاملAn O-phosphotransferase catalyzes phosphorylation of hygromycin A in the antibiotic-producing organism Streptomyces hygroscopicus.
The antibiotic hygromycin A (HA) binds to the 50S ribosomal subunit and inhibits protein synthesis in gram-positive and gram-negative bacteria. The HA biosynthetic gene cluster in Streptomyces hygroscopicus NRRL 2388 contains 29 open reading frames, which have been assigned putative roles in biosynthesis, pathway regulation, and self-resistance. The hyg21 gene encodes an O-phosphotransferase wi...
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A plasmid conferring resistance to the aminocyclitol antibiotic hygromycin B was isolated from Escherichia coli. The gene conferring resistance to this drug was cloned in pBR322, and the gene was localized to a fragment of ca. 1,510 base pairs. Resistance to hygromycin B is determined by an aminocyclitol phosphotransferase that modifies hygromycin B and structurally related antibiotics. The spe...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1982
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.35.527